ring finger domain 1 uhrf1 antibody Search Results


ring finger domain 1 uhrf1 antibody  (Proteintech)
93
Proteintech ring finger domain 1 uhrf1 antibody
<t>UHRF1</t> binds to RasGRP3 and promotes the ubiquitination of RasGRP3. ( A ) Schematic workflow of IP followed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis for identifying RasGRP3-interacting proteins in human aortic endothelial cells (HAECs). ( B ) GO enrichment analysis of proteins that specifically bind to RasGRP3. UHRF1 protein expression in isolated aortic endothelial cells from atherosclerosis mice ( C ) and oxidized low-density lipoprotein-induced human primary aortic endothelial cells ( D ) was detected using Western blot ( n = 3). ( E ) Co-Immunoprecipitation (Co-IP) between UHRF1 and RasGRP3 in human primary aortic endothelial cells showed that UHRF1 binds to RasGRP3 ( n = 3). ( F ) The protein expression levels of UHRF1 and RasGRP3 by Western blot ( n = 3). ( G ) The protein expression of RasGRP3 at different time points following Cycloheximide (CHX) treatment ( n = 3). ( H ) Co-IP assay was used to detect RasGRP3 ubiquitination. Data are present as means ± standard deviation. ## P < 0.01, ** P < 0.01, compared to the siNC + CHX group.
Ring Finger Domain 1 Uhrf1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ring+finger+domain+1+uhrf1+antibody/pmc12957022-57-66-74?v=Proteintech
Average 93 stars, based on 1 article reviews
ring finger domain 1 uhrf1 antibody - by Bioz Stars, 2026-08
93/100 stars
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ubiquitin  (Proteintech)
96
Proteintech ubiquitin
<t>UHRF1</t> binds to RasGRP3 and promotes the ubiquitination of RasGRP3. ( A ) Schematic workflow of IP followed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis for identifying RasGRP3-interacting proteins in human aortic endothelial cells (HAECs). ( B ) GO enrichment analysis of proteins that specifically bind to RasGRP3. UHRF1 protein expression in isolated aortic endothelial cells from atherosclerosis mice ( C ) and oxidized low-density lipoprotein-induced human primary aortic endothelial cells ( D ) was detected using Western blot ( n = 3). ( E ) Co-Immunoprecipitation (Co-IP) between UHRF1 and RasGRP3 in human primary aortic endothelial cells showed that UHRF1 binds to RasGRP3 ( n = 3). ( F ) The protein expression levels of UHRF1 and RasGRP3 by Western blot ( n = 3). ( G ) The protein expression of RasGRP3 at different time points following Cycloheximide (CHX) treatment ( n = 3). ( H ) Co-IP assay was used to detect RasGRP3 ubiquitination. Data are present as means ± standard deviation. ## P < 0.01, ** P < 0.01, compared to the siNC + CHX group.
Ubiquitin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ring+finger+domain+1+uhrf1+antibody/pmc12957022-57-62-74?v=Proteintech
Average 96 stars, based on 1 article reviews
ubiquitin - by Bioz Stars, 2026-08
96/100 stars
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β actin antibody  (Proteintech)
97
Proteintech β actin antibody
<t>UHRF1</t> binds to RasGRP3 and promotes the ubiquitination of RasGRP3. ( A ) Schematic workflow of IP followed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis for identifying RasGRP3-interacting proteins in human aortic endothelial cells (HAECs). ( B ) GO enrichment analysis of proteins that specifically bind to RasGRP3. UHRF1 protein expression in isolated aortic endothelial cells from atherosclerosis mice ( C ) and oxidized low-density lipoprotein-induced human primary aortic endothelial cells ( D ) was detected using Western blot ( n = 3). ( E ) Co-Immunoprecipitation (Co-IP) between UHRF1 and RasGRP3 in human primary aortic endothelial cells showed that UHRF1 binds to RasGRP3 ( n = 3). ( F ) The protein expression levels of UHRF1 and RasGRP3 by Western blot ( n = 3). ( G ) The protein expression of RasGRP3 at different time points following Cycloheximide (CHX) treatment ( n = 3). ( H ) Co-IP assay was used to detect RasGRP3 ubiquitination. Data are present as means ± standard deviation. ## P < 0.01, ** P < 0.01, compared to the siNC + CHX group.
β Actin Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ring+finger+domain+1+uhrf1+antibody/pmc12957022-57-79-83?v=Proteintech
Average 97 stars, based on 1 article reviews
β actin antibody - by Bioz Stars, 2026-08
97/100 stars
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Image Search Results


UHRF1 binds to RasGRP3 and promotes the ubiquitination of RasGRP3. ( A ) Schematic workflow of IP followed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis for identifying RasGRP3-interacting proteins in human aortic endothelial cells (HAECs). ( B ) GO enrichment analysis of proteins that specifically bind to RasGRP3. UHRF1 protein expression in isolated aortic endothelial cells from atherosclerosis mice ( C ) and oxidized low-density lipoprotein-induced human primary aortic endothelial cells ( D ) was detected using Western blot ( n = 3). ( E ) Co-Immunoprecipitation (Co-IP) between UHRF1 and RasGRP3 in human primary aortic endothelial cells showed that UHRF1 binds to RasGRP3 ( n = 3). ( F ) The protein expression levels of UHRF1 and RasGRP3 by Western blot ( n = 3). ( G ) The protein expression of RasGRP3 at different time points following Cycloheximide (CHX) treatment ( n = 3). ( H ) Co-IP assay was used to detect RasGRP3 ubiquitination. Data are present as means ± standard deviation. ## P < 0.01, ** P < 0.01, compared to the siNC + CHX group.

Journal: Inflammation

Article Title: RasGRP3 Promotes RAP1B Activity to Inhibit Endothelial Inflammation and Alleviate Atherosclerosis in High-Fat Diet-Fed ApoE −/− Mice

doi: 10.1007/s10753-026-02473-y

Figure Lengend Snippet: UHRF1 binds to RasGRP3 and promotes the ubiquitination of RasGRP3. ( A ) Schematic workflow of IP followed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis for identifying RasGRP3-interacting proteins in human aortic endothelial cells (HAECs). ( B ) GO enrichment analysis of proteins that specifically bind to RasGRP3. UHRF1 protein expression in isolated aortic endothelial cells from atherosclerosis mice ( C ) and oxidized low-density lipoprotein-induced human primary aortic endothelial cells ( D ) was detected using Western blot ( n = 3). ( E ) Co-Immunoprecipitation (Co-IP) between UHRF1 and RasGRP3 in human primary aortic endothelial cells showed that UHRF1 binds to RasGRP3 ( n = 3). ( F ) The protein expression levels of UHRF1 and RasGRP3 by Western blot ( n = 3). ( G ) The protein expression of RasGRP3 at different time points following Cycloheximide (CHX) treatment ( n = 3). ( H ) Co-IP assay was used to detect RasGRP3 ubiquitination. Data are present as means ± standard deviation. ## P < 0.01, ** P < 0.01, compared to the siNC + CHX group.

Article Snippet: Following antibodies were used: RasGRP3 antibody (1∶1000, A7791#, ABclonal Technology, Wuhan, China), vascular cell adhesion molecule-1 (VCAM-1) antibody (1∶1000, DF6082#, Affinity, Changzhou, China), RAP1B antibody (1∶500, A24472#, ABclonal Technology, Wuhan, China), p-IKK antibody (1∶1000, 2697#, Cell Signaling Technology, Danvers, MA, USA), IKK antibody (1∶1000, AF6014#, Affinity, Changzhou, China), p-IκBα antibody (1∶1000, AF2002#, Affinity, Changzhou, China), IκBα antibody (1∶1000, AF5002#, Affinity, Changzhou, China), Ubiquitin-like containing PHD and RING finger domain 1 (UHRF1) antibody (1∶1000, 21402-1-AP#, Proteintech Group, Rosemont, IL, USA), β-actin antibody (1∶20000, 66009-1-Ig#, Proteintech Group, Rosemont, IL, USA), goat anti-rabbit IgG-HRP (1∶3000, SE134#, Solarbio, Beijing, China) and goat anti-mouse IgG-HRP (1∶10000, SE131#, Solarbio, Beijing, China).

Techniques: Ubiquitin Proteomics, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Expressing, Isolation, Western Blot, Immunoprecipitation, Co-Immunoprecipitation Assay, Standard Deviation